| Name | Pos | Hgt | Wt | Birthday | Exp | College | |
| 81 | Baker, Dallas | WR | 6-3 | 206 | 11/10/82 | 1 | Florida |
| 16 | Batch, Charlie | QB | 6-2 | 216 | 12/5/74 | 11 | Eastern Michigan |
| 11 | Bloom, Jeremy | FL | 5-9 | 180 | 4/2/82 | 2 | Colorado |
| 48 | Boyd, Cody | TE | 6-8 | 264 | 10/22/83 | 2 | Washington State |
| 69 | Capizzi, Jason | RT | 6-9 | 315 | 6/19/85 | 2 | Indiana (Pa.) |
| 23 | Carter, Tyrone | SS | 5-9 | 195 | 3/31/76 | 9 | Minnesota |
| 25 | Clark, Ryan | FS | 5-11 | 205 | 10/12/79 | 7 | Louisiana State |
| 74 | Colon, Willie | RT | 6-3 | 315 | 4/9/83 | 3 | Hofstra |
| 55 | Cooper, Marquis | ROLB | 6-3 | 225 | 3/11/82 | 5 | Washington |
| 44 | Davenport, Najeh | HB | 6-1 | 247 | 2/8/79 | 7 | Miami |
| Davis, Bruce | LB | 6-2 | 252 | 9/2/85 | R | UCLA | |
| 38 | Davis, Carey | FB | 5-10 | 225 | 3/27/81 | 2 | Illinois |
| 88 | Dekker, Jonathan | TE | 6-5 | 250 | 5/15/83 | 2 | Princeton |
| Dixon, Dennis | QB | 6-3 | 206 | 1/11/85 | R | Oregon | |
| 93 | Eason, Nick | LDE | 6-3 | 305 | 5/29/80 | 6 | Clemson |
| 79 | Essex, Trai | LT | 6-4 | 324 | 12/5/82 | 4 | Northwestern |
| 51 | Farrior, James | LILB | 6-2 | 243 | 1/6/75 | 12 | Virginia |
| 50 | Foote, Larry | RILB | 6-1 | 239 | 6/12/80 | 7 | Michigan |
| 57 | Fox, Keyaron | LB | 6-3 | 235 | 1/23/82 | 5 | Georgia Tech |
| 54 | Frazier, Andre | ROLB | 6-5 | 255 | 6/29/82 | 4 | Cincinnati |
| 22 | Gay, William | LCB | 5-10 | 190 | 1/1/85 | 2 | Louisville |
| 98 | Hampton, Casey | NT | 6-1 | 325 | 9/3/77 | 8 | Texas |
| 97 | Harrison, Arnold | LOLB | 6-3 | 241 | 9/20/82 | 3 | Georgia |
| 92 | Harrison, James | ROLB | 6-0 | 242 | 5/4/78 | 5 | Kent State |
| 62 | Hartwig, Justin | C | 6-4 | 312 | 11/21/78 | 7 | Kansas |
| 34 | Haynes, Verron | HB | 5-10 | 222 | 2/17/79 | 7 | Georgia |
| Hills, Tony | OT | 6-6 | 305 | 11/4/84 | R | Texas | |
| 76 | Hoke, Chris | NT | 6-2 | 305 | 4/6/76 | 8 | Brigham Young |
| 10 | Holmes, Santonio | SE | 5-11 | 189 | 3/3/84 | 3 | Ohio State |
| Humpal, Mike | LB | 6-3 | 244 | 2/17/85 | R | Iowa | |
| 99 | Keisel, Brett | RDE | 6-5 | 285 | 9/19/78 | 7 | Brigham Young |
| 68 | Kemoeatu, Chris | RG | 6-3 | 344 | 1/4/83 | 4 | Utah |
| 90 | Kirschke, Travis | LDE | 6-3 | 298 | 9/6/74 | 12 | UCLA |
| 35 | Kreider, Dan | FB | 5-11 | 255 | 3/11/77 | 9 | New Hampshire |
| 42 | Latsko, Billy | FB | 5-10 | 233 | 2/16/84 | 1 | Florida |
| 67 | Lentz, Matt | OT | 6-6 | 320 | 11/19/82 | 1 | Michigan |
| 45 | Lorello, Mike | SS | 5-11 | 208 | 10/15/84 | 1 | West Virginia |
| 37 | Madison, Anthony | LCB | 5-9 | 180 | 10/8/81 | 3 | Alabama |
| 61 | Mahan, Sean | C | 6-3 | 301 | 5/28/80 | 6 | Notre Dame |
| 41 | Mason, Grant | LCB | 6-0 | 192 | 8/18/83 | 2 | Michigan |
| 95 | McBean, Ryan | LDE | 6-5 | 290 | 4/23/84 | 2 | Oklahoma State |
| 20 | McFadden, Bryant | RCB | 6-0 | 190 | 11/21/81 | 4 | Florida State |
| Mendenhall, Rashard | RB | 5-10 | 224 | 6/19/87 | R | Illinois | |
| 83 | Miller, Heath | TE | 6-5 | 256 | 10/22/82 | 4 | Virginia |
| 21 | Moore, Mewelde | RB | 5-11 | 209 | 7/24/82 | 5 | Tulane |
| Mundy, Ryan | S | 6-1 | 205 | 2/11/85 | R | West Virginia | |
| 39 | Parker, Willie | HB | 5-10 | 209 | 11/11/80 | 5 | North Carolina |
| 65 | Parquet, Jeremy | LT | 6-6 | 321 | 4/11/82 | 3 | Southern Mississippi |
| 71 | Paxson, Scott | NT | 6-4 | 292 | 2/3/83 | 1 | Penn State |
| 43 | Polamalu, Troy | SS | 5-10 | 207 | 4/19/81 | 6 | Southern California |
| 03 | Reed, Jeff | PK | 5-11 | 225 | 4/9/79 | 7 | North Carolina |
| 15 | Reid, Willie | FL | 5-10 | 186 | 9/19/82 | 3 | Florida State |
| 49 | Retkofsky, Jared | LS | 6-5 | 260 | 3/16/83 | 1 | TCU |
| 07 | Roethlisberger, Ben | QB | 6-5 | 241 | 3/2/82 | 5 | Miami (Ohio) |
| 33 | Russell, Gary | HB | 5-11 | 215 | 9/8/86 | 2 | Minnesota |
| 09 | Sepulveda, Daniel | P | 6-3 | 230 | 1/12/84 | 2 | Baylor |
| 73 | Simmons, Kendall | RG | 6-3 | 315 | 3/11/79 | 7 | Auburn |
| 91 | Smith, Aaron | LDE | 6-5 | 298 | 4/9/76 | 10 | Northern Colorado |
| 27 | Smith, Anthony | FS | 5-11 | 192 | 9/20/83 | 3 | Syracuse |
| 77 | Smith, Marvel | LT | 6-5 | 321 | 8/6/78 | 9 | Arizona State |
| 89 | Spaeth, Matt | TE | 6-7 | 270 | 11/24/84 | 2 | Minnesota |
| 72 | Stapleton, Darnell | C | 6-3 | 285 | 9/21/85 | 2 | Rutgers |
| 78 | Starks, Max | RT | 6-8 | 337 | 1/10/82 | 5 | Florida |
| Sweed, Limas | SE | 6-4 | 212 | 12/25/84 | R | Texas | |
| 24 | Taylor, Ike | LCB | 6-1 | 191 | 5/5/80 | 6 | La.-Lafayette |
| 94 | Timmons, Lawrence | ROLB | 6-1 | 234 | 5/14/86 | 2 | Florida State |
| 26 | Townsend, Deshea | RCB | 5-10 | 190 | 9/8/75 | 11 | Alabama |
| 18 | Trannon, Matt | FL | 6-6 | 235 | 7/22/83 | 1 | Michigan State |
| 48 | Trucks, Anthony | LB | 6-1 | 230 | 12/1/83 | 1 | Oregon |
| 28 | Vincent, Justin | RB | 5-10 | 219 | 1/25/83 | 1 | Louisiana State |
| 19 | Walker, Gerran | WR | 5-10 | 185 | 10/2/83 | 1 | Lehigh |
| 86 | Ward, Hines | FL | 6-0 | 205 | 3/8/76 | 11 | Georgia |
| 60 | Warren, Greg | LS | 6-3 | 252 | 10/18/81 | 4 | North Carolina |
| 85 | Washington, Nate | FL | 6-1 | 185 | 8/28/83 | 4 | Tiffin |
| 56 | Woodley, LaMarr | LOLB | 6-2 | 265 | 11/3/84 | 2 | Michigan |
| 05 | Zabransky, Jared | QB | 6-2 | 219 | 12/4/83 | 1 | Boise State |
